|Class 1- Introduction to the gEssential Bioinformatics for Global Leader Ih classes and general bioinformatics concept [style: lecture, discussion]
History, general definition/overview of bioinformatics and its applications in different fields of science.
Class 2- Basics of molecular biology I [style: lecture, discussion]
Introduction to the cell, organization and composition, cells in their context (models of cells, cancer, infection, development, tissue renewalc)
Class 3- Basics of molecular biology II [style: lecture, discussion, computer practice]
PROTEIN, structure/function, analytical techniques and tools, proteomics databases exploration
Class 4- Basics of molecular biology III [style: lecture, discussion, computer practice]
DNA, structure, basic genetic mechanisms (RNA, mutation, SNP, gene repair, replication, protein translation, evolutionc..), analytical techniques and tools, genomics databases exploration.
Class 5- Basics of molecular biology IV [style: lecture, discussion]
Omics and Bioinformatics; new molecular techniques, application and social implication of biological investigation.
Class 6- RT-qPCR technique and primers design [style: lecture, discussion, computer practice]
Class 7-8- RNA isolation [style: wet lab]
RNA extraction from animal tissue (Guanidinium thiocyanate-phenol-chloroform method), RNA quantitation assessment (Nanodrop)
Class 9- RNA quality assessment [style: wet lab]
Class 10- Gene expression analysis I [style: wet lab]
Class 11-12- Gene expression analysis II [style: computer practice, discussion]
RT-PCR data analysis-Example of small scale high throughput transcriptomics method (PCR array)
Class 13-14- Protein expression analysis I [style: wet lab, computer practice, discussion]
Semi-quantitative Western-Blotting experiment(gel electrophoresis, immunoblotting)
Class 15- Protein expression analysis II [style: wet lab, computer practice, discussion]
Semi-quantitative Western-Blotting, data analysis (Image J)